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  1. Molecule Type (DNA/RNA). mfold gets sequence information from command line arguments. Information about what type will be passed to the tool can be seen next to the sequence name
  2. Molecule Topology (linear/circular). mfold gets sequence information from command line arguments. If the sequence is marked as circular, then mfold will work with it as a circular one. Otherwise, as with linear one.
    Whether the sequence is linear/circular is described here.
    Part of a circular sequence will also be considered a circular sequence. If you want to change this behavior, uncheck "Mark sequence as circular" and then call the mfold dialog.
  3. Anchor
    #internal-dpi
    #internal-dpi
    DPI of images in UGENE
    . Ghostscript has a default DPI of 72. Therefore, this value is used for images for the internal UGENE report and this value cannot be changed.

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  1. Status, Time,  At the beginning there is information about the run: information about the task, settings for launch.
  2. Output HTML report. Clickable link to the output HTML report. More about this report in the next section.
  3. Found structures. List of found structures. The structure is characterized by free energy. When clicked, UGENE will scroll the window to the desired structure.
  4. Anchor
    structure-table
    structure-table
    Structure table
    . The  For each found structure, the table contains:
    1. Thermodynamic details: free energy, enthalpy, entropy and an estimated Tm.
    2. Loop Free-Energy Decomposition: the entire decomposition of the particular folding into loops and stacks, together with their free energies and closing base pairs. Consecutive runs of base pairs are summarized as helices.
    3. Image. Displays of nucleic acid secondary structure. At the bottom of the image there is information identifying it: free energy, name of the input sequence and analyzed region. It has the above-mentioned fixed DPI of 72.
  5. mfold log. Information about launching the external mfold tool: launch command and log.

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