...
The melting temperature is calculated as follows. For sequences of length 15 or longer:
Tm = 64.9 + 41 * (nG + nC - 16.4) / (nA + nT + nG + nC) |
For shorter sequences:
Tm = (nA + nT) * 2 + (nG + nC) * 4 |
Here "nA", "nT", "nC", "nG" denote the number of the corresponding nucleotide.
Context information about a sequence can be found on the All information is contextual, i.e. it shows statistics about the currently selected region (on the selected sequence). The tab includes information about:
...
nmole/OD260
TODO: add description/meaning?
nmole/OD260 = 1000000 / molarExtCoef
μg/OD260
TODO: add description/meaning?
μg/OD260 = nmoleOD260 * molarWeight * 0.001;
Amino acid sequence common statistics
...