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To make a request to a local BLAST database do the following:

  • Open Tools ‣ BLAST + ‣ BLAST + Searchsearch.

If there is a sequence opened you can also initiate the request to a local BLAST database from the Sequence View:

  •  Select the Analyze ‣ Query with BLAST+ item item in the context menu or in the Actions the main menu.

The Request to local BLAST + database dialog will appear:

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