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All available parameters are the same as in the original Primer3. Move
NOTE: The "Primer Designer" dialog has a detailed description of each parameter - just move the mouse cursor to the parameter you're interested about (or its name) to see a and the tooltip appears. Due to this fact, there are no detailed description of in this parameterfile.
However there is one additional feature available which is not originally a part of Primer3 tool. It allows user design primers for RT-PCR experiments by choosing which exons/introns to span with the primer product. This feature is described in detailed below. When you select the parameters you can save and load settings with a help of the corresponding buttons in the right corner of the dialog.
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