Child pages
  • RNA-seq Analysis with Tuxedo Tools

Versions Compared

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.

The RNA-seq pipeline “Tuxedo” consists of the TopHat spliced read mapper, that internally uses  Bowtie or Bowtie 2 short read aligners, and several Cufflinks tools that allows one to assemble transcripts, estimate their abundances, and tests for differential expression and regulation in RNA-Seq seq samples.

...

Warning
titleEnvironment Requirements

The pipeline is currently available on Linux and Mac OS X systems only.

Tip
titleHow to Use This Sample

If you haven't used the workflow samples in UGENE before, look at the "How to Use Sample Workflows" section of the documentation.

 Select Samples tab on the Workflow Designer Palette and double-click on the ChIP-seq analysis with Cistrome tools sample. The following configure wizard appears:

...

As soon as the variants calling task is finished, a notification and dashboard will appear. The dashboard will contain information about workflow: input and output files, all information about task.

 

Info

The work on this pipeline was supported by grant RUB1-31097-NO-12 from NIAID.