Unipro UGENE Online User Manual v. 1.15.0
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  • Primers Details

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For Click Show primers details for seeing statistic details about primers click Show primers details.  
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<center>
<br>
  <img src="/wiki/download/attachments/13435071/Primers Details.png"/>
</br>
</center>

The following dialog will appear:

HTML
<center>
<br>
  <img src="/wiki/download/attachments/13435071/Primers Details_1.png"/>
</br>
</center>

This is a dialog with statistic details about primers: melting temperature, GC content, dimers, hairpinsself-dimers, etc. If a value is not correct for its criteria then it is written with the colored in red color.