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  • Align Sequences with MUSCLE

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  1. Input MSA(s): On this page you must input multiple alignments file(s). 

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  2. Align Sequences with MUSCLE: On this page you can modify MUSCLE and output parameters. 

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    The following parameters are available:

    ModeSelector of preset configurations, that give you the choice of optimizing accuracy, speed, or some compromise between the two. The default favors accuracy.
    Stable order

    Do not rearrange aligned sequences (-stable switch of MUSCLE).

    Otherwise, MUSCLE re-arranges sequences so that similar sequences are adjacent in the output file. This makes the alignment easier to evaluate by eye.

    Max iterationsMaximum number of iterations.

    Region to align

    Whole alignment or column range e.g. 1..100.

    Result alignment

    Location of output data file. If this attribute is set, slot "Location" in port will not be used.

    Document format

    Document format of output file.