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The algorithm applied in RT-PCR primer design first searches for all available primers in a given sequence. Then it filters the detected pairs to make sure that they satisfy the selected configuration. This option allows to set the maximum number of pairs for the initial search query. Larger number will result in increased sensitivity, but also in a longer running time. Default value is 1000.
Note, that Important: using the RT-PCR primer design tab will reset the values set in the Exlcuded regions and Targets of the Main configuration tab. Additionally if the Exon range option is set, the defined sequence region will be ignored.
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