The workflow scans each input sequence from the end to find the first position where the quality is greater or equal to the minimum quality threshold. Then it trims the sequence to that position. If a the whole sequence has quality less than the threshold or the length of the output sequence less than the minimum length threshold then the sequence is skipped.
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If you haven't used the workflow samples in UGENE before, look at the "How to Use Sample Workflows" section of the documentation. |
Workflow Sample Location
The workflow sample "FASTQ Trimmer" can be found in the "Custom Elements" section of the Workflow Designer samples.
Workflow Image
The workflow looks as follows:
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<center> <br> <img src="/wiki/download/attachments/16122713/FASTQ Trimmer.png"/> <br> </center> |