...
The Find restriction sites dialog appears:
The tree of restriction enzymes
...
The region to search enzymes in. If you check the Uncut area checkbox,. You may also Exclude some region and not to search in itthan restriction enzymes, which exists outside of the uncut area, but also presisted inside of it, won't be included in the result.
Example:
You will find two "AsaI" enzymes in the following sequence if you choose the whole sequence as Search in:
But, if you also set Uncut area to 60-90 you will not find enzymes at all and will see the following sing in the log: The following enzymes were found, but skipped because they are presented inside of the "Uncut area": Aasi.
The information about enzymes was obtained from the REBASE database. For each enzyme in the list a brief description is available (the accession ID in the database, the recognition sequence, etc.). If you’re online you can get more detailed information about an enzyme selected by clicking the REBASE Info button.
...