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Finds annotations for the supplied DNA sequence in local BLAST database.

Warning
BLAST is used as an external tool from UGENE and it must be installed on your system. To learn more about the external tools, please, read main UGENE User Manual.

Parameters in GUI

Parameter	Description	Default value
Search type	Selects the type of the BLAST searches.	blastn
Database path	Path to the database files.
Database name	Base name for BLAST DB files.
Tool path	Path to the BLAST executable.	default
Temporary directory	Directory for temporary files.	default
Expected value	Expectation threshold value.	10
Best hits limit	Specifies the number of best hits from a region of the query to keep. 0 turns it off. If used, 100 is recommended.	0
Annotate as	Name of the result annotations.	blast_result
Gapped alignment	Perform gapped alignment.	use
Gap costs	Cost to create and extend a gap in an alignment.	2 2
Match scores	Reward and penalty for matching and mismatching bases.	1 -3
BLAST output	Location of BLAST output file.
BLAST output type	Type of BLAST output file.	XML (-m 7)

Parameters in Workflow File

Type: blast

Parameter	Parameter in the GUI	Type
blast-type	Search type	string Available values are: blastn blastp blastx tblastn tblastx
db-path	Database path	string
db-name	Database name	string
tool-path	Tool path	string
temp-dir	Temporary directory	string
e-val	Expected value	numeric
max-hits	Best hits limit	numeric
result-name	Annotate as	string
gapped-aln	Gapped alignment	boolean
gap-costs	Gap costs	string
match-scores	Match scores	string
blast-output	BLAST output	string
type-output	BLAST output type	string

Input/Output Ports

The element has 1 input port:

Name in GUI: Input sequence

Name in Workflow File: in-sequence

Slots:

Slot In GUI	Slot in Workflow File	Type
Sequence	sequence	sequence

And 1 output port:

Name in GUI: Annotations

Name in Workflow File: out-annotations

Slots:

Slot In GUI	Slot in Workflow File	Type
Set of annotations	annotations	annotation-table

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