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Melting temperature
Click on the gear icon near Melting temperature sign (marked as hyperlink) label and the Melting temperature settings dialog will be opened.
The melting temperature is calculated as follows. For sequences of length 15 or longer:
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Tm = 64.9 + 41 * (nG + nC - 16.4) / (nA + nT + nG + nC)
For shorter sequences:
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Tm = (nA + nT) * 2 + (nG + nC) * 4
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Note, that UGENE computes Tm fot sequence regions from 8 to 10000 bp. Otherwise, an undefined value N/A is output.
See the corresponding page for settings details.
nmole/OD260
The amount of DNA or RNA represented in nanomoles per 1 unit of absorbance at 260 nm dissolved in 1 ml cuvette with 1 cm pathlength.
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