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UGENE provides the In silico PCR feature only for nucleic sequences. To use it in UGENE open a DNA sequence and go to the In Silico silico PCR tab of the Options Panel: 

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Type two primers for running In Silico PCR. If the primers pair is invalid for running the PCR process then the warning is shown. Also, primers for the running In Silico silico PCR can be chosen from a primers primer library. Click the following button to choose a primer from the primers library: 

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HTML
<center>
<br>
  <img src="/wiki/download/attachments/13435073/Primers Library_1.png"/>
</br>
</center>

 The table consists of the following columns: name, GC-content (%), Tm, Length (bp) and sequence. Select primer in the table and click on the Choose button. 

Click the Reverse-complement button for making a primer sequence reverse-complement: 

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When you run the process, the predicted PCR products appear in the products table.

 Products table

There are three columns in the table:

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