In silicoPCR searches a seuence database with a pair of PCR primers, using an indexing strategy for fast perfomance. In silico PCR is available only for nucleic sequences. To use it in UGENE open a DNA sequence and go to the In Silico PCR tab of the Options Panel:
| HTML |
|---|
<center> <br> <img src="/wiki/download/attachments/13435069/In Silico PCR.png"/> </br> </center> |
...
Here the following parameters are available:
Forward primer - forward primer.
Reverse primer - on the opposite strand from the forward primer.
Mismatches - mismatches.
Maximum product - maximum size of amplified region.