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Type two primers for running In Silico PCR. If the primers pair is invalid for running the PCR process then the warning is shown. Also, primers for the running In Silico PCR can be chosen from a primers library.  To Click the following button to choose a primer from the primers library click on the following button: 

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  <img src="/wiki/download/attachments/13435073/Primers Library.png"/>
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 The following dialog will appear:  

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  <img src="/wiki/download/attachments/13435073/Primers Library_1.png"/>
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 The table consists of the following columns: name, GC-content (%), Tm, Length (bp) and sequence. Select primer in the table and click on the Choose button. 

Click the Reverse-complement button for making a primer sequence reverse-complement: 

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