To consider the sequence as circular and be able to search for restriction sites between the end and the beginning of the sequence check the Circular molecule option.
Example: Let’s consider:
- The sequence is “CTGC ... CAC”.
- AarI restriction enzyme (with recognition sequence “CACCTGC”) has been checked.
In this case if the Circular molecule option has been checked, the restriction site will be found. If it hasn’t been checked, the restriction site won’t be found (in this position).