The 'mfold' software is a tool for nucleic acid folding and hybridization prediction. It was developed by M. Zuker in the late 1980s for RNA folding and improved for DNA folding in 1996.

Tool home page: mfold.org. Similar results to those produced by UGENE can be obtained using the mfold web server (DNA prediction, RNA prediction) or using the OligoAnalyzer tool. For complete documentation on the tool, see a) on the website, b) in the article, c) in the source code in the corresponding folder.

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Usage

mfold only works with DNA or RNA sequences. You can call the dialog from
the Global Sequence View actions 
or Main Menu→Actions→Analyze 
or the context sequence menu→Analyze  

Tool comparison