Primers for the running In Silico PCR can be chosen from a primers library. Primers library it is a storage for keeping user primers. The added primers are stored between UGENE sessions.
To choose a primer from the primers library click on the following button:
<center> <br> <img src="/wiki/download/attachments/13435073/Primers Library.png"/> </br> </center> |
The following dialog will appear:
<center> <br> <img src="/wiki/download/attachments/13435073/Primers Library_1.png"/> </br> </center> |
The table consists of the following columns: name, GC-content (%), Tm, Length (bp) and sequence. Select primer in the table and click on the Choose button.
To configure primers library go to the Tools->Primer->Primer library context menu. The following window will appear:
<center> <br> <img src="/wiki/download/attachments/13435073/Primers Library_2.png"/> </br> </center> |