The Primer3 plugin is a port of the Primer3 tool. It is intended to pick primers from a DNA sequence.
To use the Primer3, open a DNA sequence and select the Analyze ‣ Primer3 context menu item. The dialog will appear:
<center> <br> <img src="/wiki/download/attachments/4227733/Primer3.png"/> <br> </center>
All available parameters are the same as in the original Primer3.
However there is one additional feature available which is not originally a part of Primer3 tool. It allows user design primers for RT-PCR experiments by choosing which exons/introns to span with the primer product. This feature is described in detailed below. When you select the parameters you can save and load settings with a help of the corresponding buttons in the right corner of the dialog.