Align reads with BWA-MEM

Performs alignment of short reads with BWA-MEM.

Parameters in GUI

Parameter	Description	Default value
Output directory	Directory to save BWA-MEM output files.
Reference genome	Path to indexed reference genome.
Output file name	Base name of the output file. 'out.sam' by default.	out.sam
Number of threads	Number of threads (-t).	1
Min seed length	Path to indexed reference genome (-k).	19
Band width	Band width for banded alignment (-w).	100
Dropoff	Off-diagonal X-dropoff (-d).	100
Internal seed length	Look for internal seeds inside a seed longer than {-k} (-r).	1.50000
Skip seed threshold	Skip seeds with more than INT occurrences (-c).	10000
Drop chain threshold	Drop chains shorter than FLOAT fraction of the longest overlapping chain (-D).	0.5
Rounds of made rescues	Perform at most INT rounds of mate rescues for each read (-m).	100
Skip mate rescue	Skip mate rescue (-S).	False
Skip pairing	Skip pairing; mate rescue performed unless -S also in use (-P).	False
Mismatch penalty	Score for a sequence match (-A).	1
Mismatch penalty	Penalty for a mismatch (-B).	4
Gap open penalty	Gap open penalty (-O).	6
Gap extension penalty	Gap extension penalty; a gap of size k cost {-O} (-E).	1
Penalty for clipping	Penalty for clipping (-L).	5
Penalty unpaired	Penalty for an unpaired read pair (-U).	17
Score threshold	Minimum score to output (-T).	30

Parameters in Workflow File

Type: bwamem-id

Input/Output Ports

The element has 1 input port:

Name in GUI: BWA data

Name in Workflow File: in-data

Slots:

Slot In GUI	Slot in Workflow File	Type
URL of a file with mate reads	readsurl	string
URL of a file with reads	readspairedurl	string

And 1 output port:

Name in GUI: BWA-MEM output data

Name in Workflow File: out-data

Slots:

Slot In GUI	Slot in Workflow File	Type
Assembly URL	assembly-out	string