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Primers for the running In Silico PCR can be chosen from a primers library. Primers library it The primer library is a storage for keeping user primers. The added primers are stored between UGENE sessions.

To choose a primer from the primers library click on the following button:

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The following dialog will appear:

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  <img src="/wiki/download/attachments/13435073/Primers Library_1.png"/>
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The table consists of the following columns: name, GC-content (%), Tm, Length (bp) and sequence. Select primer in the table and click on the Choose button. 

To configure primers library go  Go to the Tools->Primer->Primer library context menu to configure the primer library. The following window will appear: 

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  <img src="/wiki/download/attachments/13435073/Primers Library_2.png"/>
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To Click the New primer button to add a new primer click on the New primer button. The following dialog will appear: 

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  <img src="/wiki/download/attachments/13435073/Primers Library_3.png"/>
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Input the primer sequence and primer name and click on the OK button. To remove primer(s) from the library select it

Select the primer in the table (you can use Ctrl and Shift) and then click click Remove primer(s) button . To edit primer select it and click on the to remove primer(s). 

Select the primer and click the Edit primer button to edit primer.