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<center> <br> <img src="/wiki/download/attachments/65930783/Primer Library_3.png"/> </br> </center> |
Add Select Add file(s) button for adding one or several files with primer sequences. Note that all sequence formats, supported by UGENE, can be imported, for example, FASTA, GenBank, etc. But the sequences must consist of ACGT characters only.
Select Add object(s) button for adding one or several items already loaded in the Project.
Click the Import button to import the added files or objects into the primers library.
Click the Temperature button for choosing temperature calculation algorithm: Rough or Primer 3.
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<center> <br> <img src="/wiki/download/attachments/65930783/Primer Library_4.png"/> </br> </center> |