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In silicoPCR searches a seuence database with a pair of PCR primers, using an indexing strategy for fast perfomance. In silico PCR is available only for nucleic sequences. To use it in UGENE open a DNA sequence and go to the In Silico PCR tab of the Options Panel: 

Here the following parameters are available:

Forward primer - forward primer.

Reverse primer - on the opposite strand from the forward primer. 

Mismatches - mismatches.

Maximum product - maximum size of amplified region.


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