This feature allows to span corresponding intron sequences on the genomic sequence or junction sequences between exons.
Note that this feature is only available for mRNA/cDNA sequences with annotated exons. There are several ways to obtain the cDNA for a corresponding DNA sequence.
- From NCBI or ENSEMBLE database
- Align the genomic and cDNA sequences using spliced aligner
To use this feture go to the RT-PCR tab of the Primer Designer dilaog:
The following parameters are available:
Exon annotation name - exon annotation name.
Span exon-exon junction - minimum exon junction overlap size.
Exon range - exon range.
Span at least one intron: the default mode.
Max numbers of pairs to query - max numbers of pairs to query.